Life Processes &
the Chemistry of Life
After four topics on rock and time, the course turns to biology. The question is older than science itself: what counts as alive? Marine scientists answer it with seven simple tests — and a deeper second layer: every one of those tests runs on the same handful of chemicals that have powered life on Earth for 3.5 billion years.
A submersible ROV exploring the abyssal plain off the Cape of Good Hope spots a pale grey pebble sitting on the seabed. The camera operator on the ship above isn't sure: is it just a rock, or could it be alive? She can't poke it (it's 5 km down). She can't smell it. She can't taste it. How does she decide?
By running through seven tests. Every biology textbook on Earth — from Cape Town to Tokyo — uses the same seven. They spell out MRS GREN: Movement, Respiration, Sensitivity, Growth, Reproduction, Excretion, Nutrition. If the pebble shows even most of these processes, it's alive. If it shows none, it's a rock. (Pyura stolonifera — Red Bait — looks exactly like a pebble until you watch it squirt water at you.)
But MRS GREN is just the top layer. Underneath, every one of those processes is powered by chemistry — by water acting as the universal solvent, by minerals doing structural and catalytic work, and by four families of organic macromolecules (carbohydrates, lipids, proteins, nucleic acids) doing everything from storing energy to carrying the hereditary instructions that built every cell. This topic is the bridge from "what is life?" to "what is life made of?" — and every biology topic that follows builds on it.
Two Floors of Biology
Life can be described at two levels: as seven processes any organism must perform (MRS GREN), and as the chemistry that makes those processes possible.
Start with the definition of the subject itself, because CAPS opens this block with it. Biology — also called Life Sciences — is the study of all living things. That includes the organisms themselves, the environment they live in, and their interactions both with that environment and with each other. It is a wider definition than "the study of animals and plants", and the width is the point: a marine biologist studying how a kelp forest responds to an upwelling event is doing biology just as much as one dissecting a fish.
At the macroscopic level, anything alive must do seven things: move parts of itself, get energy from organic substances, react to its surroundings, grow, make more of itself, get rid of chemical waste, and obtain the chemicals it needs to do all of the above. These are the seven life processes — Mrs Gren. They're the universal definition of "alive".
At the microscopic level, every one of those processes is just chemistry: water dissolving compounds inside cells, minerals doing structural and catalytic work, and four big families of organic molecules — carbohydrates, lipids, proteins, nucleic acids — doing the actual heavy lifting. A whale and a phytoplankton cell run on the same chemistry. So does a kelp blade and a coral polyp. This shared chemistry is why biology works as one subject.
The Seven Life Processes — Mrs Gren
Tap each letter to see what the process means, why it matters, and where you'd spot it in a marine organism. Get the mnemonic into your head — you will use it in every biology topic from here on, and it will appear in exams.
~ the seven things every living thing does
There is an eighth criterion, and it is the door into the next topic
MRS GREN gives you seven processes. CAPS adds one more requirement, and it is easy to miss because it is written inside the brackets of criterion 6: "Excretion and other chemical and metabolic processes (and the organism is made up of cellular units governed by nucleic acids)."
Your booklet states the same thing more plainly, as an eighth criterion: every living organism consists of one or more membrane-bound cellular units, governed by a set of nucleic acids. So a thing is not alive merely because it does seven things — it must also be built a particular way.
📌 This is the sentence that makes Topic 14 necessary. It is also the cleanest reason a virus is such an awkward case: it carries nucleic acids but is not a cell and cannot run the seven processes on its own. You will meet that argument properly in Topic 16.
Local connection — apply MRS GREN at the rock pools
Walk the rocky shore at Cape Recife (Gqeberha) or St James (False Bay) at low tide and you can tick off MRS GREN on almost any creature you find. A Red Bait tunicate (Pyura stolonifera) looks like a brown rock — but poke it and it squirts water (sensitivity + movement). A limpet grazes algae off the rock (nutrition + locomotion). A sea anemone closes when you touch it (sensitivity). All of them respire, excrete waste into the water and reproduce by releasing eggs and sperm into the tides. The Two Oceans Aquarium in Cape Town — the same organisation that wrote your curriculum — is the easiest place in South Africa to see every one of these processes happening in real time.
The Chemistry Floor — Inorganic Foundations
Before we get to the big organic molecules, two inorganic essentials underpin everything: water and minerals. Without these two, none of the life processes above would be possible.
Water — the universal solvent
The water molecule is polar covalent: oxygen pulls the shared electrons slightly towards itself, making the oxygen side slightly negative (δ−) and the hydrogen sides slightly positive (δ+). This split charge is called a dipole.
The dipole makes water spectacular at dissolving things. When an ionic compound like salt (NaCl) hits water, the negative oxygen ends pull the Na⁺ ions apart, while the positive hydrogen ends pull on the Cl⁻ ions. The compound dissociates — and now the dissolved ions can react. That's why every cell in every living thing has water as its inside medium.
Minerals — taken up as compounds, never as elements
Read that heading carefully, because CAPS makes a point of it. Organisms do not absorb calcium, or iron, or magnesium. They absorb inorganic ions and dissolved compounds — calcium ions, nitrate, phosphate — and it is the element inside the compound that gets used as a building material or to enable a chemical reaction. A mussel does not eat calcium off the sea floor; it takes up dissolved Ca²⁺ ions from seawater and lays them down as calcium carbonate shell.
The same substances, taken up the same way, are what a farmer or a marine botanist calls nutrients — the word changes with the subject, the chemistry does not. In plants and algae the uptake is the whole basis of growth: nitrate and phosphate are the limiting nutrients in the sea, which is why an upwelling that lifts them into the sunlit layer sets off a phytoplankton bloom (Topic 4).
Those needed in larger amounts are macronutrients; those needed in tiny amounts are micronutrients, also called trace elements.
The body of your Topic 13 booklet defines minerals biologically — "inorganic ions which are absorbed in relatively small amounts…normally absorbed as compounds or dissolved ions and not as elements". That is the definition this section teaches, and it is the one CAPS uses.
But the booklet's examinable definitions list at the back gives a completely different, geological one: "inorganic crystalline material obtained by mining…mostly found in rocks." That is a fine definition of a mineral in geology — and it is the wrong one for this topic, where nothing is being mined. In a Life Sciences answer, use the biological definition. If you are asked to reproduce the glossary definition, give it and then say which sense you are using; being able to spot that one word carries two meanings in two subjects is worth more than either definition alone.
Macronutrients
Calcium
Bones, teeth and the shells & skeletons of marine organisms (snails, urchins, corals).
Sodium
Maintains fluid balance; vital for nerve impulses and muscle contraction.
Potassium
Works with sodium across cell membranes — same nerve & muscle role.
Phosphorus
Sits inside ATP (the energy molecule), inside nucleic acids, and inside many proteins.
Magnesium
A key catalyst in respiration and photosynthesis. Sits at the centre of every chlorophyll molecule.
Sulphur
Found in many amino acids and proteins; locks protein shapes in place.
Micronutrients (trace elements)
Copper
Enables many enzymes to drive metabolic processes.
Zinc
Similar enzyme-enabling role to copper.
Iron
Sits inside haemoglobin, the red pigment that carries oxygen in blood.
The Big Five — Organic Compounds
An organic compound is any chemical containing carbon covalently bonded to hydrogen, usually with oxygen as well, and often nitrogen and phosphorus too. What makes carbon special is what it does next: complex organic compounds are built on chains or rings of carbon atoms, with the other elements hung off them. That skeleton is why organic molecules can be enormous and endlessly varied while still being stable — and it is the single sentence that explains why life is carbon-based rather than built on anything else.
CAPS names five things cells are made of and contain: proteins, carbohydrates, lipids, nucleic acids and vitamins. Learn them as five. It is tempting to treat vitamins as an afterthought because they are needed in tiny amounts, but the syllabus lists them alongside the other four and examines them.
⚠️ The definition of "organic" is not exact, and CAPS says so. There are exceptions at the edges — carbon dioxide and carbonates contain carbon but are counted as inorganic, and a very few organic compounds have no hydrogen at all. Do not let an edge case distract you from the working rule: C bonded to H, in chains or rings.
Carbohydrates
Monomer: monosaccharide (glucose, fructose, galactose)
Energy & structure, in three levels. Monosaccharides (glucose, fructose, galactose) are soluble — instant energy. Disaccharides (sucrose, maltose) are two joined together, still soluble — short-term storage. Polysaccharides (starch, glycogen) are large and insoluble — long-term storage. Cellulose is a longer chain still, and is structural in plants.
~ glucose · sucrose · starch · cellulose
Lipids (fats & oils)
Building blocks: glycerol + 3 fatty acids
Storage, insulation, structure. Long-term energy storage. Thermal insulation — the blubber on a whale or seal. Shock cushioning around organs. And every cell membrane in every organism is made of lipids.
~ blubber · cell membranes · nerve insulation
Proteins
Monomer: amino acid (50+ make a polypeptide)
The biological workhorses. Structural (skin, scales, muscle, cartilage), recognition (antibodies, cell-surface markers), transport (haemoglobin carries O₂), control (hormones), and catalysis (enzymes drive every cellular reaction).
~ enzymes · hormones · haemoglobin · antibodies
Nucleic acids
Monomer: nucleotide (sugar + N-base + phosphate)
Hereditary information. DNA stores the instructions for building every protein in the organism; RNA reads them out and helps the cell execute. Every characteristic an organism inherits passes through these molecules.
~ DNA · RNA · the genetic code
Vitamins
No single monomer — a mixed group defined by what they do
Regulation. Complex organic compounds needed in tiny amounts that regulate mineral uptake, regulate growth and cell division, assist specific enzyme-catalysed reactions, and act as antioxidants. Deficiency causes disease; excess of a stored one causes hypervitaminosis.
~ A · D · E · K (fat-soluble) · B · C (water-soluble)
Your booklet says cellulose is "formed by plants as an important structural material", and that is the wording to reproduce. Be careful applying it to seaweed: kelp is a brown alga, not a plant, and its cell walls are cellulose fibres together with alginates (alginic acid) — which is what Topic 24 teaches and what gives kelp its rubbery flexibility in surf. Saying "cellulose is what kelp cell walls are made of" is half an answer.
Recognising the molecules on sight
Your booklet sets this as an outcome in as many words: "be able to specifically identify glucose, fructose, an amino acid and a simple fat from molecular diagrams." That means a paper can put a structure in front of you with no label and ask what it is. You are not expected to memorise every bond — you are expected to know the give-away feature of each one. Tap a molecule to see what to look for.
Enzymes — and the half of the definition most learners miss
An enzyme is a protein that acts as a catalyst: it initiates and controls a specific chemical reaction without being used up or altered. Almost every reaction inside a living organism is run by one.
🔴 Here is the part that is usually taught wrong. Enzymes are almost always introduced as things that speed up or break down — digestion, and that is it. But read CAPS: "Discuss the role of enzymes in breaking down or synthesising molecules." Enzymes do both directions, and the two directions have names you are expected to know.
Together these two make up metabolism — the sum total of every chemical reaction happening inside an organism. An organism that could only do catabolic reactions would digest itself and never grow. That is why "enzymes speed up digestion" is half a definition, and why the word CAPS uses is synthesising.
Temperature works on enzymes in two different ways — and only one is denaturing
Enzymes function best inside a narrow temperature range and a narrow pH range. Outside it they stop working — but how they stop depends on which direction you went, and this is a mark learners routinely lose.
❄️ Too cold → the enzyme becomes inactive. The molecules have too little energy to collide and react. The enzyme has not been damaged: its shape is intact, and warming it back up restores full function. This is reversible.
🔥 Too hot (or extreme pH) → the enzyme is denatured. The chain unfolds, the precise 3D shape is destroyed, and the target molecule no longer fits. This is permanent. Cooling it down again does nothing.
📌 So: write "inactive" for cold and "denatured" for heat. Writing "the enzyme was denatured" for a chilled sample is wrong, and it is an easy mark to lose in a cold-water marine course where the question could plausibly be about either. Cook an egg to watch denaturing happen — the clear albumin turns solid white and never goes back. Put an egg in the fridge and nothing has been destroyed at all.
This is exactly why thermoregulation matters so much: an organism that cannot hold its temperature steady starts losing enzyme function across the board. It is why whales and seals evolved blubber, and why fish are restricted to particular temperature bands.
Eat too much protein and you make a poison — the ammonia–urea chain
This is the one place the chemistry half of this topic joins the MRS GREN half, and a CAPS paper likes joins.
If the diet is short of energy compounds, or if more protein is eaten than the body needs, the excess protein is broken down (a catabolic reaction) and used for its chemical potential energy or stored. But protein contains nitrogen, and the carbohydrates and fats it is being substituted for do not. That nitrogen has to go somewhere, and it comes off as ammonia — which is toxic.
The body converts the ammonia to the far less toxic urea, and excretes it. So: protein → ammonia → urea → excretion. That is why urea appears on the excretion list at all, and it is the answer to "why do animals excrete nitrogen but not carbon?" — carbon leaves as CO₂ through respiration; nitrogen has no such route.
Vitamins — the fifth constituent
Vitamins are complex organic compounds needed in only tiny amounts, but a deficiency causes potentially life-threatening disorders, and in some cases too much causes harm as well — hypervitaminosis. They do four jobs: regulate mineral uptake, regulate growth and cell division, assist specific enzyme-catalysed processes, and act as antioxidants.
The split that matters is solubility, because it decides how often you have to eat them:
| Group | Vitamins | Stored in the body? | Consequence |
|---|---|---|---|
| Fat-soluble | A · D · E · K | Yes — held in fatty tissue | Do not need eating daily. But because they accumulate, these are the ones that can cause hypervitaminosis. |
| Water-soluble | B group · C | No — excreted easily | Must be eaten regularly. Also easily lost from food that is frozen or overcooked, so how you cook changes what you actually absorb. |
CAPS sets this one as homework — do it, it is two marks in an exam
The Investigations column for this block asks for a short research task: "the name and function of 2 or 3 each of water soluble and fat soluble Vitamins." Naming the groups is not enough — you need to be able to say what an individual vitamin actually does.
The task, with a worked table to check yourself against, is Investigation 4 on the practical sheet. There is a strong marine angle to it too: vitamin D is made in skin using sunlight, which raises an obvious question about animals that never surface into strong light — and vitamin C deficiency is scurvy, the disease that shaped centuries of sea voyaging and killed more sailors than storms did.
One more organic molecule sits slightly outside CAPS's list of five but underpins all of them:
- ATP (Adenosine Triphosphate) — the unstable, short-lived energy-carrying molecule produced by respiration. Every life process that needs energy spends ATP. It is the reason respiration sits underneath all six of the other life processes.
In the Lab — the CAPS Investigations
This block carries the only practical investigations CAPS sets in the whole Chemistry of Life section, and they are the classic ones: test a food for carbohydrate, for lipid, and for protein. Each test is a colour change, and each colour change is a mark.
What CAPS asks for, word for word
Investigations column, block 4: "Practical investigations · Test for carbohydrates · Test for lipids · Test for proteins" and "Homework research — the name and function of 2 or 3 each of water soluble and fat soluble Vitamins". Resources column: "Chemicals for food tests" — the only resource CAPS lists for the whole four-hour half. The one-hour half adds "Classify organisms into living and non-living".
All of it is on the practical sheet. Two of the reagents genuinely cannot be bought for a home kitchen, so those two ship with a supplied result set instead — you answer exactly the same analysis questions either way.
| Testing for | Reagent | Method | Positive result |
|---|---|---|---|
| Starch a polysaccharide |
Iodine solution | Add a few drops to the sample | Orange-brown → blue-black |
| Reducing sugars glucose, fructose, maltose |
Benedict's solution | Add and heat in a water bath | Blue → green → yellow → orange → brick-red, the further the more sugar |
| Protein | Biuret reagent | Add and shake — no heating | Blue → violet / purple |
| Lipids | Ethanol, then water or just paper |
Emulsion test, or rub on paper | Cloudy white emulsion, or a translucent spot that will not dry out |
The trap in the sugar test — and it is a favourite
Benedict's detects reducing sugars. Glucose, fructose and maltose are reducing sugars and give the colour change. Sucrose is not — table sugar gives a negative Benedict's result even though it is unmistakably a sugar.
So a negative Benedict's does not mean "no sugar present". It means "no reducing sugar present". The way to catch sucrose is to break it into its two monosaccharides first and then re-test — and watching a negative turn positive after that step is the best thing this practical can teach you about how a chemical test actually works.
Five investigations · two need the supplied data, three do not
- 🟡 pharmacy Test for carbohydrates — iodine for starch across a set of foods, then the reducing-sugar test with a supplied Benedict's result set (and a glucose test-strip substitute if you can get one).
- 🟢 kitchen Test for lipids — the grease-spot test needs nothing but paper, and the emulsion test needs methylated spirits. Fully doable at home.
- 🔴 lab only Test for proteins — biuret reagent cannot be safely made in a kitchen, so this one ships as a described result set. Every analysis question still works.
- 🟢 desk Vitamin research — CAPS's homework task: name and function of 2–3 water-soluble and 2–3 fat-soluble vitamins, with a worked table to check against.
- 🟢 desk 🌊 shore Living or non-living? — the one-hour half's investigation, run as a MRS GREN classification on real and awkward cases.
→ Open the Topic 13 practical sheet — printable, with supplied data for every task.
Key Vocabulary
Tap each card to flip it. Your booklet prints a list headed "these definitions are examinable and integral to your understanding of the topic", and that list has thirty-one terms on it — by some distance the longest in the course so far. All thirty-one are below. There is no way to make that a short section, and pretending otherwise by showing you eight of them would not be doing you a favour.
The first eight are the ones that carry the distinctions CAPS asks about most — respiration vs breathing, excretion vs egestion, autotrophic vs heterotrophic. The remaining twenty-three then run in the booklet's own alphabetical order, so you can revise straight down the page against the list at the back of your book.
⚠️ Watch anabolic / catabolic (build up vs break down) and macronutrients / micronutrients (larger amounts vs smaller amounts). Both pairs differ by one syllable and mean opposite things. And see the warning in Section three about minerals, where your booklet's glossary definition disagrees with its own body text.
The cellular process by which an organic substance (usually glucose) is chemically converted to release energy — captured as ATP — for life functions. Not the same as breathing, which is the gas exchange of getting oxygen into the body and CO₂ out.
Getting rid of chemical waste from cellular metabolism — CO₂, urea, ammonia, salt. Not the same as egestion, which is the expulsion of undigested food as faeces. Excretion gets rid of waste the body made; egestion gets rid of waste the body never absorbed.
Any change in the internal or external environment of an organism — light, temperature, touch, sound, smell, chemical balance. Organisms with sensitivity detect stimuli and react. A sea anemone closing when you touch it has detected a tactile stimulus.
Able to produce its own energy-rich food from inorganic compounds and an external energy source — plants and algae do this with photosynthesis (light energy); some deep-sea bacteria do it with chemosynthesis (chemical energy). Opposite: heterotrophic, organisms that must eat other organisms.
A compound containing carbon covalently bonded to hydrogen (and often oxygen, nitrogen, phosphorus too). Includes all carbohydrates, lipids, proteins and nucleic acids. Inorganic compounds — like water and salt — can contain carbon (e.g. CO₂) but never C and H together.
A chemical that speeds up a reaction without being used up by it. In biology, almost all catalysts are enzymes (a kind of protein). One enzyme can catalyse millions of reactions before wearing out.
What happens when a protein is exposed to high heat or extreme pH — it loses its precise 3D shape and stops working. The egg white going from clear to white as you cook it is denaturing in action. Why thermoregulation matters so much for living organisms.
The building block of nucleic acids. Each one is a sugar (deoxyribose in DNA, ribose in RNA) bonded to a nitrogen base and a phosphate group. Strung together by the millions, they form the DNA and RNA molecules that carry genetic information.
The monomers of proteins. There are 21 protein-forming amino acids; the number, combination and order of them decides which protein you get, and therefore its shape and its job. Each contains C, H, O and N — and it is that nitrogen that later has to leave as ammonia and urea.
Chemical processes enabling the production of larger, more complex compounds from smaller ones — building up. Joining amino acids into a protein is anabolic. It costs energy. Opposite of catabolic.
Very important proteins in animal immune systems which enable them to recognise, then target and destroy invasive pathogens. They work by shape-matching, which is why they are proteins and why heat destroys them.
Adenosine Triphosphate — the unstable energy-carrying molecule within most cells performing life processes. Made by respiration, spent by everything else. "Unstable" is the whole point: it gives its energy up easily, so it cannot be stockpiled.
Chemical processes in which complex compounds are broken into smaller ones — breaking down, e.g. digestion. It releases energy. Opposite of anabolic. Respiration is catabolic.
The process in which a cell develops from an unspecialised cell to a more specialised one — changing its structure so it can do a specific job. It is how one fertilised egg becomes an organism with eyes, gills and muscle.
The separation of an ionically bonded molecule into two or more ions in solution. Salt dissociating into Na⁺ and Cl⁻ in water is the example — and it only happens because water is a dipole. Once dissociated, the ions can react.
The process whereby an animal gets rid of undigested waste material in the form of faeces. Not excretion. Egestion removes what was never absorbed; excretion removes what the body's own metabolism made.
Proteins which act as catalysts in organic processes, facilitating both anabolic and catabolic reactions. Note the word both — an enzyme that only broke things down would be half a definition, and CAPS says "breaking down or synthesising".
Information passed from adults to offspring in the form of genetic material, which dictates their physical makeup and functioning. Carried by the nucleic acids — DNA and RNA.
Unable to produce its own food, and therefore relying on external sources of organic compounds from dead or living organisms. Every fish, whale, shark, squid and human. Opposite of autotrophic.
Organic compounds produced and released within an organism to trigger a response elsewhere within it — often proteins. They regulate growth, sexual maturation, metabolic rate and the response to danger. They find their target because membrane proteins recognise them.
An adverse reaction or condition due to taking in too much of a specific vitamin. It is mostly a risk with the fat-soluble vitamins (A, D, E, K), because those are the ones the body stores rather than excretes.
All elements and compounds which do not have a combination of Carbon and Hydrogen, or chains of Carbon, in them. Water and salts are inorganic. CO₂ contains carbon but no C–H bond, so it counts as inorganic too.
Chemical compounds needed in larger amounts. In this topic: Ca, Na, K, P, Mg and S. Do not confuse with micronutrients — one syllable apart, opposite meaning.
The sum total of all chemical reactions taking place within an organism — anabolic and catabolic together. Respiration, photosynthesis, digestion and muscle contraction are all metabolic processes, and all of them generate the waste that excretion has to remove.
Chemical compounds needed in smaller amounts, also called trace elements. In this topic: Cu, Zn and Fe. "Trace" describes the quantity, not the importance — no iron means no haemoglobin.
Biological sense (use this one): inorganic ions absorbed in relatively small amounts, taken up as compounds or dissolved ions, never as elements, and used as structural components or to enable chemical processes.
⚠️ Your booklet's glossary gives a geological definition instead — "inorganic crystalline material obtained by mining…mostly found in rocks". That is the right definition in Topic 10 and the wrong one here. See the flag in Section three.
The chemical building blocks of larger compounds. Monosaccharides are the monomers of carbohydrates, amino acids of proteins, nucleotides of nucleic acids. Learn the word and three examples together — it is a one-mark question.
Large, complex molecules made up of monomers (nucleotides) consisting of a sugar bonded to a nitrogen base and a phosphate group. They occur as DNA and RNA, and store and transfer hereditary information.
A combination of a large number of amino acids bonded together — normally more than 50, at which point it is called a protein. Two joined amino acids make a dipeptide; many make a polypeptide.
A fluid in which other molecules, known as solutes, can dissolve to form solutions. Water is the biological solvent, and it is good at the job because it is a dipole — which is the link straight back to Section three.
The ability to regulate and maintain a favourable body temperature. It matters because enzymes go inactive when too cold and are denatured when too hot — so an organism that cannot hold its temperature loses enzyme function across the board.
Quick Check
Question 1
Well done!
Next: Cell Biology — zoom in further on how the chemistry of life is organised inside the smallest living unit.